Microarray analysis of the developing rat mandible.

نویسندگان

Hideo Kaburagi

Naoyuki Sugano

Maiko Oshikawa

Ryosuke Koshi

Naoki Senda

Kazuhiro Kawamoto

Koichi Ito

چکیده

To analyze the molecular events that occur in the developing mandible, we examined the expression of 8803 genes from samples taken at different time points during rat postnatal mandible development. Total RNA was extracted from the mandibles of 1-day-old, 1-week-old, and 2-week-old rats. Complementary RNA (cRNA) was synthesized from cDNA and biotinylated. Fragmented cRNA was hybridized to RG-U34A GeneChip arrays. Among the 8803 genes tested, 4344 were detectable. We identified 148 genes with significantly increased expression, and 19 genes with significantly decreased expression. A comprehensive analysis appears to be an effective method of studying the complex process of development.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Developing a Filter-Wrapper Feature Selection Method and its Application in Dimension Reduction of Gen Expression

Nowadays, increasing the volume of data and the number of attributes in the dataset has reduced the accuracy of the learning algorithm and the computational complexity. A dimensionality reduction method is a feature selection method, which is done through filtering and wrapping. The wrapper methods are more accurate than filter ones but perform faster and have a less computational burden. With ...

متن کامل

Extracellular exosomes and preeclampsia: a microarray-based study and functional enrichment analysis

Background: Preeclampsia (PE) is a heterogeneous pregnancy disease which the exact pathophysiology of it is unknown. Recently exosomes have been indicated as a causative factor in the pathogenesis of PE. The aim of the study was to investigate in microarray library data to extract the differentially expressed genes (DEGs) in PE and to perform a functional enrichment analysis to predict the rol...

متن کامل

Valproic Acid-Induced Time Dependent Craniofacial Defects in Wistar Rat Fetuse

Purpose: we previously reported that maternal valproic acid (VA) administration during rat pregnancy produced CNS defect ranely, syringomyelia. Furthermore, it seems that administration of valproic acid during critical period of pregnancy may affect on development of other embryonic skeletal portion such as craniofacial region. The goal of our study was to determine whether there is a relations...

متن کامل

تحلیل تصاویر ریزآرایه به منظور تشخیص نوع سرطان سینه

Background: Microarray technology is a powerful tool to study and analyze the behavior of thousands of genes simultaneously. Images of microarray have an important role in the detection and treatment of diseases. The aim of this study is to provide an automatic method for the extraction and analysis of microarray images to detect cancerous diseases. Methods: The proposed system consists of t...

متن کامل